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All these lessons are available in downloadable .pdf files that you can store on your hard drive and access whenever you wish. These lessons are interspersed with a variety of other links that make available many other scientific articles of interest, as well as videos that can be viewed online or stored to your hard drive again.
You will need to download Adobe Reader to be able to view all these lessons.
Try a couple of SAMPLE COURSES BELOW:
SAMPLE LESSON 1 SAMPLE LESSON 2
SYLLABUS
Lesson 1 – Introduction to Live Blood Analysis
This lesson discusses some of the advantages and disadvantages of darkfield microscopy, as well as some of the basic functions of magnification and resolution. It also introduces some of the concepts of ‘Visual Medicine’ and what can be seen and interpreted in this fascinating world of darkfield microscopy.
 Lesson 2 – The Compound Microscope
This lesson covers the ‘Anatomy and Physiology’ of your microscope, including the different parts, the physics of light and colour, the objectives, a brief history of microscopy from the 16th to the 21st century, ‘how does it work,’ oculars and eyepieces, magnification and how this works, lenses and geometric optics, tube length and magnification, illumination, Darkfield Microscopy – the workings of the condensers, low and high magnification, setup and troubleshooting, oil immersion procedures, cleaning, care and maintenance, fundamentals of video imaging and photomicrography. The lesson is interspersed with many Interactive Java Tutorials as well as videos and other references.
Lesson 3 – Blood Work: Taking Samples Correctly
Taking the blood sample correctly is crucially important as this can reflect on the accuracy of the interpretation – it is possible to damage the RBCs and WBCs during collection. There are basic materials and guidelines in this lesson to guide the student into standardizing their blood taking for both live and dry blood analyses – this is crucial for their own safety as well as for optimizing the interpretation of the blood picture.
Lesson 4 – Louis Pasteur Vs Antoine Béchamp and The Germ Theory of Disease Causation
Traditional Western medicine teaches and practices the doctrines of French chemist Louis Pasteur (1822-1895). Pasteur's main theory is known as the Germ Theory Of Disease. It claims that fixed species of microbes from an external source invade the body and are the first cause of infectious disease. The concept of specific, unchanging types of bacteria causing specific diseases became officially accepted as the foundation of allopathic Western medicine and microbiology in late 19th century Europe. Also called monomorphism,(one-form), it was adopted by America's medical/industrial complex, which began to take shape near the turn of the century. These issues are discussed in detail with the work of Royal Raymond Rife, Biological Terrain Analysis, Virology, Immunology and Fungi and Moulds all placed in a historical context.
 Lesson 5 – The Father of Darkfield Microscopy: Prof. Enderlein
This is an important lesson as it lays down the groundwork of Prof. Enderlein’s initial work at the beginning of the century. There are a number of links to original papers by Prof. Enderlein himself, published in his book entitled “Bacteria Cyclogeny” – it is advisable to take the time to study this material even though it appears to be ‘heavy going’ at first. You may need to read the material two and three times over to really get a grasp on it – this is quite the norm for the majority of students so do not think that you are not bright enough to understand it, as it is really a different ‘language’ that requires time to understand and assimilate. The lesson includes a lot of original reading material from Prof. Enderlein himself, as well as videos of interest to watch.
Lesson 6 – Identifying the Basic Blood Forms in Live Blood
This lesson will take us through the components of blood – given that this is the medium that you will be working with under the darkfield microscope. The basic blood compositions are examined in detail such as plasma, plasma proteins and their functions, platelets and their functions, red blood cells and white blood cells and their functions, and blood groups. There are also many Interactive Java Tutorials that look at RBC animations, the circulatory system as well as many downloadable .pdf files consisting of over 250 pages of text.
Lesson 7 – The Oxidative Stress Test (Dry Blood Analysis)
This lesson will examine in detail Dry Blood Analysis which is also referred to as the Oxidative Stress Test (OST). In the 1920s European medical practitioners added another twist to unconventional microscopy when they began looking at dried blood samples, later called the Oxidative Stress Test. A glass microscope slide is dabbed onto a bead of blood on the finger in sequence several times, resulting in a slide with 8 individual drops of blood pressed upon the slide and allowed to air dry.
The resulting patterns seen in the dry blood under the bright field format reveal a characteristic "footprint" which can be seen in similar cases and, thus, are predictive of certain generalised pathologies. For instance, cases of advanced degenerative disease show very poor clotting, minimal fibrin formation with many white "puddles" disseminated throughout the sample. A healthy control subject's blood shows a tight, fibrin rich clotting pattern with no white puddles.
 Lesson 8 – What Are We Seeing In Live Blood Analysis: Interpreting Blood Forms
In this lesson we will examine how we interpret the various blood morphologies that we see under darkfield – what do all these mean? Prof. Enderlein spent many years observing morphologies under the microscope and indeed in those days the technology was much less developed that it is today. Most darkfield microscopists usually study the Enderleinian perspective and analyze the blood according to this.
Modern microbiologists do not recognize the morphologies or the language used by Enderlein and as expert witnesses in a court of law, their word counts. However, there is the more traditional, scientific approach that microbiologists would understand and agree with the various morphologies seen in darkfield – here you will be on safe, scientific ground as you are simply using your darkfield microscope as a scientist identifying various morphologies and interpreting according to traditional microbiological knowledge.
In this course the intention is not to omit either of these two views – if anything, the Enderlein perspective has important historical significance, but on the other hand science is not set in stone and I strongly believe that more work in this field of live blood analysis will reveal a lot that is not part of our knowledge base at the moment. Therefore, Enderlein’s perspective will be given, along with a number of his own papers for those that want to pursue his research further and get a better understanding. Most of these papers are in Lessons 4 and 5 which talks about the history of darkfield microscopy as well as the work of Prof. Enderlein.
Lesson 9 – Zeta Potentials: Explaining the Development of the Morphologies
In this lesson we will examine how live blood analysis works – how are the various morphologies developed and why. In order to understand how this works we need to look at the ZETA POTENTIAL, a common concept in physics. The zeta potential is a measure of the magnitude of the repulsion or attraction between particles. Simply put, Zeta Potential is a measurement of the charge on the surface of blood cells, platelets, proteins and debris in the plasma. It is the force responsible for particles in blood repelling one another or clumping together.
 Lesson 10 – Parasites: The Uninvited Guests!
One of the reasons why we have included this lesson on parasites is because often you will see various parasites under the darkfield or phase contrast microscope. Identifying them is an art and science in itself, and this requires deepening your knowledge in parasitology as well as “training the eye” to be able to identify what you are seeing. This is intended to be an introductory lesson in parasitology in the hope that you will arm ourselves with more specialized books on the subject and begin really identifying what you see.
Lesson 11 – Fungi and Moulds: Feeding off the Dead!
It is often possible to see various fungi and mould under darkfield, so this is a useful section to include. There are many people that suffer from fungal and mould infections and treatment protocols are included to address these issues.
Lesson 12 – Time Lapse Decay (TLD)
There are many practitioners that examine a blood sample for no more than 10-15 minutes and conclude from this. If you spend time looking at blood samples you will see many changes taking place over time – usually a number of hours are required. This is what has been termed ‘Time Lapse Decay’ or TLD – a brief introduction to this will be provided to familiarize yourself with the concept and understand the significance of ‘behavioural microscopy.’
Final Exams
Once all the modules are completed there will be final exams to complete – students must score 60% and above to obtain a pass – 80% and above obtains a distinction. Students who do not pass the exam with a minimum of 60% will be required to re-register for the exam and take it again. All exams are timed on-line and scores will be given automatically.
OWNING A DARKFIELD MICROSCOPE
There are many microscopes that are advertised on the Web which are purported to be suitable for Live Blood Analysis work. These microscopes can range between $3,000 up to over $20,000 depending on the specifications. Many people have bought these microscopes, even top-of-the-range ones, only to find that they were not suitable for darkfield studies.
It is therefore important that the beginner or average professional who is beginning their studies in Live Blood Analysis know what they are buying. Dr. Georgiou has carefully chosen a microscope that gives excellent value for money at the lower end of the price spectrum. It is a high-quality laboratory microscope that is very versatile and will give a life-times service with no problems if looked after properly.
Click on the links below if you want to study the specifications - there are two models to choose from, but if you can choose the AL3000-DF150 model this is the one to really go for as the picture quality is astounding.
LOMO DARKFIELD MICROSCOPE BROCHURE
LOMO MICROSCOPE DETAILED SPECIFICATIONS
BUY YOUR LIVE BLOOD ANALYSIS MICROSCOPE
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